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KMID : 1007520150240041447
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Food Science and Biotechnology
2015 Volume.24 No. 4 p.1447 ~ p.1451
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Isolation, Expression, and Characterization of S-adenosyl-L-methionine Synthetase from Lactobacillus paraplantarum 72815
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Cha In-Tae
Nam Young-Do
Seo Myung-Ji
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Abstract
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The S-adenosyl-L-methionine (SAM) synthetase gene was mined from Lactobacillus paraplantarum 72815 isolated from the traditional Korean fermented food Kimchi. The gene was cloned and expressed in Escherichia coli to obtain recombinant SAM synthetase (SAM-s-LP). The purified SAM-s-LP was characterized as 40°C and pH 8.5 as the optimal temperature and pH for activity, respectively. The apparent Km values of SAM-s-LP were 1.47±0.59 mM for L-Met and 2.84±0.82 mM for ATP.
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KEYWORD
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S-adenosyl-L-methionine synthetase, characterization, expression, Lactobacillus paraplantarum
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